Isolation and Biochemical Identification of N2 Fixing Bacteria (Azospirillium Sp.) From Saurashtra Region

Azospirillium species are widely used as Biofertilizer to increase crop yield and free-living nitrogen-fixing bacteria, one of the best-studied plant growth-promoting Rhizobacteria. It is commonly found along with roots many different species. The present study included isolation from soils in Rhizospheric regions. 50 isolates were isolated fields districts Saurashtra region Gujarat against NFB Medium. Significant root surface internal tissues. These morphologically identified biochemically characterized techniques documented Bergey's Textbook Identified Bacteria 9th edition (TSI- Triple Sugar Iron, GPB- Glucose Phosphate Broth, MR- Methyl Red VP- Voges Proskauer, TRP-Tryptophan, PNB- Peptone Nitrate Carbohydrate) out which fifteen positive also capable producing IAA (Indole Acetic Acid), IAA.